Activities of deoxyribonuclease (DNase), ribonuclease (RNase) and RNase inhibitor were determined in the laryngeal cancer tissue to evaluate the use of nuclease and nuclease inhibitor as biochemical markers for laryngeal cancer. Also the enzymes
and
enzyme inhibitor in the laryngeal cancer tissue were analysed by a DEAE-cellulose column chromatography to understand the nature of laryngeal cancer.
Concentrations of RNA and protein were unchanged. But DNA content in the laryngeal cancer tissue was significantly increase. Acid DNase and RNase inhibitor activities were increased, and RNase activity was unchanged in the cancer tissue.
The positive rates of DNA content, activities of acid DNase and RNase inhibitor as markers for laryngeal cancer were high, suggesting the use of three parameters as biochemical markers for the cancer.
Proteins in the laryngeal cancer tissue were seperated by a DEAE-cellulose column chromatography into 7 peaks, of which a single protein peak was specific to the cancer. Acid DNase was seperated as single enzyme peak and the enzyme thus purified
was
highly6 active toward double stranded DNA. Neutral RNase was seperated into three isozymes (RNase isozymes Ⅳ, Ⅴ and Ⅵ), of which RNase isozymes Ⅳand Ⅴ were activated and RNase inhibitor activity associated with these two isozymes were
elevated.
These results indicated that acid DNase, RNase isozymes Ⅳ and Ⅴ and RNase inhibitors complexed with three isozymes in the laryngeal cancer tissue might play roles in carcinogenesis and suppression of laryngeal cancer. (Korean J Otolaryngol
38:10,
1995)
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